Allergy information for: Pomegranate (Punica granatum )

Name: Pomegranate
Scientific Name: Punica granatum
Occurrence:
Allergy Information:
Other Information:
Taxonomic Information: NEWT http://www.ebi.ac.uk/newt/display?from=null&search=22663
Last modified: 18 October 2006

Reviews (0)

Number of Studies:1-5
Number of Patients:1-5
Symptoms:Symptoms include oral allergy syndrome, abdominal pain, generalised or facial urticaria, anaphylactic shock, laryngeal oedema, shortness of breath, bronchospasm, tongue angioedema (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

Number of Studies:1-5
Food/Type of allergen:
Fruit (Gaig et al. 1999 [58]; Igea et al. 1991 [825]).

Fresh fruit and liquefied fruit (Gaig et al. 1992) [336]
Protocol: (controls, definition of positive etc)
Use of the prick to prick technique (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

1% histamine was used as positive control (Gaig et al. 1992) [336].
Number of Patients:
Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

A 7-year-old girl (Gaig et al. 1992) [336]

A 85-year-old woman (Igea et al. 1991) [825]
Summary of Results:
SPT was positive in the three patients (Gaig et al. 1999) [58].

SPT was positive (Gaig et al. 1992) [336].

SPT was negative after 15 min, 6 and 24 h (Igea et al. 1991) [825]

Number of Studies:0
Food/Type of allergen:
Pomegranate pulp extract (Gaig et al. 1999) [58].

Pomegranate extract (juice) (Igea et al. 1991) [825]
IgE protocol:RAST and immunoblotting (Gaig et al. 1999) [58].
RAST; Positivity was accepted when it was class 2 (0.7-3.5 PRU/ml) (Gaig et al. 1992) [336].
EIA (positivity was accepted between 4.8 and 160 IU/ml), RAST and histamine release test (Igea et al. 1991) [825]
Number of Patients:
Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

A 7-year-old girl (Gaig et al. 1992) [336]

A 85-year-old woman (Igea et al. 1991) [825]
Summary of Results:
RAST assays were positive in 2/3 patients (3.21 and 0.75 PRU/ml) (Gaig et al. 1999) [58].
RAST was positive (0.8 PRU/ml) (Gaig et al. 1992) [336]
EIA was positive but RAST and histamine release assays were negative (Igea et al. 1991) [825]

Immunoblotting separation:Protein separation was performed using 12.5% acrylamide 1D SDS-PAGE gels. Samples were reduced with beta-mercaptoethanol (Gaig et al. 1999) [58].
Immunoblotting detection method:Proteins were transferred to PVDF membranes in buffer. Membranes were blocked , incubated with sera (undiluted) and bound IgE detected by peroxidase conjugated rabbit antihuman IgE. Blots were developed by chemiluminescence (Gaig et al. 1999) [58].
Immunoblotting results:
Each of the 3 patients reacted only with a 29 kDa protein (Gaig et al. 1999) [58].

Number of Studies:1-5
Food used and oral provocation vehicle:Pomegranate seeds were crushed and mixed with 100 cl of strawberry yoghourt (Igea et al. 1991) [825]
Blind:Yes
Number of Patients:
A 85-year-old woman (Igea et al. 1991) [825]
Dose response:A single dose was given daily and the dose was increased 2-fold in the subsequent days. The challenge was positive with 40 g of pomegranate (Igea et al. 1991) [825]
Symptoms:Tongue angioedema developed 4 h after (Igea et al. 1991) [825]

Igea JM, Cuesta J, Cuevas M, Elias LM, Marcos C, Lazaro M, Compaired JA
Adverse reaction to pomegranate ingestion
Allergy. 46(6):472-4.. 1991
PUBMEDID: 1957996
Garcia J, Carreno A, Contreras L et al
Alergia a la granda. Revision de nuestra casuistica
Rev Esp Alergol Immunol Clin 11:Suppl 2 146.. 1996
PUBMEDID:
Gaig P, Botey J, Gutierrez V, pena M, Eseverri JL, Marin A
Allergy to pomegranate (Punica granatum)
J Investig Allergol Clin Immunol 2:216-218.. 1992
PUBMEDID: 1342903
Gaig P, Bartolome B, Lleonart R, Garcia-Ortega P, Palacios, R, Richart C
Allergy to pomegranate (Punica granatum)
Allergy 54:287-288.. 1999
PUBMEDID: 10321568