Allergy information for: Pomegranate (Punica granatum )

Reviews (0)

    References (0)

      Clinical History

      • Number of Studies:1-5
      • Number of Patients:1-5
      • Symptoms:Symptoms include oral allergy syndrome, abdominal pain, generalised or facial urticaria, anaphylactic shock, laryngeal oedema, shortness of breath, bronchospasm, tongue angioedema (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

      Skin Prick Test

      • Number of Studies:1-5
      • Food/Type of allergen:

        Fruit (Gaig et al. 1999 [58]; Igea et al. 1991 [825]).

        Fresh fruit and liquefied fruit (Gaig et al. 1992) [336]

      • Protocol: (controls, definition of positive etc)

        Use of the prick to prick technique (Igea et al. 1991 [825]; Gaig et al. 1992 [336]; Gaig et al. 1999 [58]).

        1% histamine was used as positive control (Gaig et al. 1992) [336].

      • Number of Patients:

        Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

        A 7-year-old girl (Gaig et al. 1992) [336]

        A 85-year-old woman (Igea et al. 1991) [825]

      • Summary of Results:

        SPT was positive in the three patients (Gaig et al. 1999) [58].

        SPT was positive (Gaig et al. 1992) [336].

        SPT was negative after 15 min, 6 and 24 h (Igea et al. 1991) [825]

      IgE assay (by RAST, CAP etc)

      • Number of Studies:0
      • Food/Type of allergen:

        Pomegranate pulp extract (Gaig et al. 1999) [58].

        Pomegranate extract (juice) (Igea et al. 1991) [825]

      • IgE protocol:RAST and immunoblotting (Gaig et al. 1999) [58].

        RAST; Positivity was accepted when it was class 2 (0.7-3.5 PRU/ml) (Gaig et al. 1992) [336].

        EIA (positivity was accepted between 4.8 and 160 IU/ml), RAST and histamine release test (Igea et al. 1991) [825]

      • Number of Patients:

        Two 25-year-old women and a 3-year-old child (Gaig et al. 1999) [58].

        A 7-year-old girl (Gaig et al. 1992) [336]

        A 85-year-old woman (Igea et al. 1991) [825]

      • Summary of Results:

        RAST assays were positive in 2/3 patients (3.21 and 0.75 PRU/ml) (Gaig et al. 1999) [58].

        RAST was positive (0.8 PRU/ml) (Gaig et al. 1992) [336]

        EIA was positive but RAST and histamine release assays were negative (Igea et al. 1991) [825]

      Immunoblotting

      • Immunoblotting separation:Protein separation was performed using 12.5% acrylamide 1D SDS-PAGE gels. Samples were reduced with beta-mercaptoethanol (Gaig et al. 1999) [58].
      • Immunoblotting detection method:Proteins were transferred to PVDF membranes in buffer. Membranes were blocked , incubated with sera (undiluted) and bound IgE detected by peroxidase conjugated rabbit antihuman IgE. Blots were developed by chemiluminescence (Gaig et al. 1999) [58].
      • Immunoblotting results:

        Each of the 3 patients reacted only with a 29 kDa protein (Gaig et al. 1999) [58].

      Oral provocation

      • Number of Studies:1-5
      • Food used and oral provocation vehicle:Pomegranate seeds were crushed and mixed with 100 cl of strawberry yoghourt (Igea et al. 1991) [825]
      • Blind:Yes
      • Number of Patients:

        A 85-year-old woman (Igea et al. 1991) [825]

      • Dose response:A single dose was given daily and the dose was increased 2-fold in the subsequent days. The challenge was positive with 40 g of pomegranate (Igea et al. 1991) [825]
      • Symptoms:Tongue angioedema developed 4 h after (Igea et al. 1991) [825]

      IgE cross-reactivity and Polysensitisation

      Other Clinical information

      Reviews (0)

        References (4)

        • Igea JM, Cuesta J, Cuevas M, Elias LM, Marcos C, Lazaro M, Compaired JA
          Adverse reaction to pomegranate ingestion
          Allergy. 46(6):472-4.. 1991
          PUBMEDID: 1957996
        • Garcia J, Carreno A, Contreras L et al
          Alergia a la granda. Revision de nuestra casuistica
          Rev Esp Alergol Immunol Clin 11:Suppl 2 146.. 1996
          PUBMEDID:
        • Gaig P, Botey J, Gutierrez V, pena M, Eseverri JL, Marin A
          Allergy to pomegranate (Punica granatum)
          J Investig Allergol Clin Immunol 2:216-218.. 1992
          PUBMEDID: 1342903
        • Gaig P, Bartolome B, Lleonart R, Garcia-Ortega P, Palacios, R, Richart C
          Allergy to pomegranate (Punica granatum)
          Allergy 54:287-288.. 1999
          PUBMEDID: 10321568