Allergy information for: Cotton seed (Gossypium herbaceum )

Clinical History

• Number of Studies:1-5
• Number of Patients:6-10
• Symptoms:

  Patient ate whole grain bread, experienced tingling and itching in mouth, angioedema and generalised urticaria (Malanin and Kalimo 1988) [407 ]

  Angioedema, urticaria, pruritis, bronchospasm, dyspnea, laryngeal oedema, rhinitis, nausea, vomiting, diarrhoea, loss of consciousness (range of symptoms experienced, all 7 patients usually experienced several symptoms at once) (Atkins et al. 1988 [269])

  Dysphagia, itching palms, wheezing, vomiting, loss of consciousness (O'Neil and Lehrer 1989 [446]).

Skin Prick Test

• Number of Studies:1-5
• Food/Type of allergen:Commercial extract and extract of foods causing a reaction with phosphate buffered saline (1:10 w/v). Extracts were filtered through 0.45µm pore filter and stored with 50% (v/v) glycerin (Atkins et al. 1988 [269]).
• Protocol: (controls, definition of positive etc)

  Histamine (1mg/ml) and saline were positive and negative controls. Wheal diameters >3mm larger than negative control were taken as positive.

• Number of Patients:9
• Summary of Results:

  Bread extract produced 8x13mm wheal with pseudopods; crushed cottonseed produced 9x18mm wheal (Malanin and Kalimo 1988 [407 ])

  7 patients with a history of reactions to products containing cottonseed protein tested were positive to cottonseed extracts and to extracts from foods containing cotton seed (Atkins et al. 1988 [269]).

  One patient who suffered a reaction after eating a candy bar on an airliner was subsequently tested against cottonseed extract and phosphate buffered saline extract of candy bar - positive to cottonseed extract (O'Neil and Lehrer 1989 [446]).

IgE assay (by RAST, CAP etc)

• Number of Studies:0
• Food/Type of allergen:
• IgE protocol:
• Number of Patients:7
• Summary of Results:Atkins et al. 1988 [269] gives only total IgE levels (6-880 IU/ml) and no data on specific IgE or immunoblotting.

Immunoblotting

• Immunoblotting separation:
• Immunoblotting detection method:
• Immunoblotting results:Not performed

Oral provocation

• Number of Studies:1-5
• Food used and oral provocation vehicle:Defatted cotton seed flour was blended into chilled vanilla flavoured Vivonex (Norwich-Eaton Pharmaceutical).
• Blind:Yes
• Number of Patients:2
• Dose response:Increasing doses of 100, 350, 500mg of cottonseed flour were given. One patient experienced symptoms after the third dose (500mg); the other after the first (Atkins et al. 1988 [269]).
• Symptoms:Chest tightness, large falls in FEV1 (forced expiratory volume in 1 second), FVC (forced expiratory vital capacity), in both patients; pharyngeal pruritis, lip swelling, nausea in one patient only (Atkins et al. 1988 [269]).

IgE cross-reactivity and Polysensitisation

Other Clinical information

Reviews (0)

References (5)

• Spies JR, Coulson EJ, Chambers DC, Bernton HS, Stevens H, Shimp JH.
  The Chemistry of Allergens. XI. Properties and Composition of Natural Proteoses Isolated from Oilseeds and Nuts by the CS-1A Procedure
  J. Am. Chem. Soc. 73(8); 3995-4001.. 1951
  PUBMEDID:
• Youle RJ, Huang AHC
  Albumin storage protein and allergens in cottonseeds.
  J Agric Food Chem 27: 500-503.. 1979
  PUBMEDID: 582177
• Atkins FM , Wilson M, Bock AS
  Cottonseed hypersensitivity: new concerns over an old problem.
  J Allergy Clin Immunol 82: 242-250.. 1988
  PUBMEDID: 3403864
• O'Neil CE, Lehrer SB
  Anaphylaxis apparently caused by a cottonseed-containing candy ingested on a commercial airliner
  J Allergy Clin Immunol 84: 407. 1989
  PUBMEDID: 2778246
• Malanin G and Kalimo K
  Angioedema and urticaria caused by cottonseed protein in whole-grain bread.
  J Allergy Clin Immunol 82: 261-264.. 1988
  PUBMEDID: 3403866

Biochemical Information for 2S albumin

• Allergen Name:2S albumin
• Alternatve Allergen Names:CS-1A
• Allergen Designation:
• Protein Family:PF00234; tryp_alpha_amyl; 2S albumin seed storage protein
• Sequence Known?:No
• Allergen accession No.s:N/A
• 3D Structure Accession No.:Not determined
• Calculated Masses:N/A
• Experimental Masses:15 kDa
• Oligomeric Masses:N/A
• Allergen epitopes:Not known
• Allergen stability:
  Process, chemical, enzymatic:Allergenicity survives heating to 100 degrees C for 1 hour in the extraction method of Spies' group.
• Nature of main cross-reacting proteins:None known
• Allergen properties & biological function:2S albumin seed storage protein.
• Allergen purification:3g of dehulled cottonseed is homogenised in 100ml of PBS for 20min. Crude extract centrifuged at 10000g for 30 min and aqueous supernatant collected. The 2S albumins were purified by sucrose density centrifugation.
• Other biochemical information:

References (2)

• Spies JR, Coulson EJ, Chambers DC, Bernton HS, Stevens H, Shimp JH.
  The Chemistry of Allergens. XI. Properties and Composition of Natural Proteoses Isolated from Oilseeds and Nuts by the CS-1A Procedure
  J. Am. Chem. Soc. 73(8); 3995-4001.. 1951
  PUBMEDID:
• Youle RJ, Huang AHC
  Albumin storage protein and allergens in cottonseeds.
  J Agric Food Chem 27: 500-503.. 1979
  PUBMEDID: 582177