Diaz-Perales et al. (1998)  reported that a single band of 32 kDa was recognized in both chestnut and avocado extracts by IgE from either a pool of the four sera or each individual serum. Purified purified chestnut and avocado class I chitinases which were also recognized and the class II chitinases were not. Preincubation with the purified class I chitinases could fully inhibit binding of IgE from the pooled sera to chestnut extracts. The N-terminal sequences of the class I chitinases were determined.
Blanco et al. (1999)  found that IgE from pooled sera of patients bound to a single band of 32 kDa in both the crude chestnut and avocado extracts. This band coincided with one from the purified chestnut and avocado class I chitinases which were also recognized, although the class II chitinases were not.
Diaz-Perales et al (1999)  found that sera from patients with latex allergy alone did not recognise allergens in chestnut extracts. Sera from patients with latex/fruit allergy recognised allergens from chestnut, cherimoya, passion fruit, kiwi, latex, papaya, mango, potato, tomato and wheat. All except the potato band were also recognised by anti-class I chitinase IgG.
Diaz-Perales et al (2002)  found bands at 31 kDa with both native and recombinant Cas s 5 which could be inhibited by the Cas s 5 preparations or by latex hevein, Hev b 6.
Diaz-Perales et al. (2000)  reported that the serum pool reactive to apple and peach LTPs, also contains specific IgE to the expected low molecular weight bands of the mugwort and chestnut extracts, as well as to the corresponding isolated LTPs. The chestnut polypeptide shows an even stronger reaction than the apple and peach allergens.