Genotyping

CIGMR provides genotyping in four main areas:

• Microsatellite typing
• SNP (single nucleotide polymorphism) typing
• MHC (major histocompatibility complex) genotyping (animals only)
• Reference Strand Conformational Analysis (RSCA)

Microsatellite genotyping on Applied Biosystems (ABI) 3100 platforms is routinely performed for human and mouse microsatellite markers.

Equipment

• High throughput SNP genotyping: currently performed using Sequenom mass spectrometry iplex technology. An Affymetrix high throughput SNP chip typing platform is also available
• Lower throughput technologies: currently still used and more appropriate for some projects include snapshot (ABI 3100), Taqman allelic discrimination (Roche LC480)
• MHC typing for companion animals includes canine DLA class II (DRBI, DQA, DQB) and class I (DLA-88) by a combination of sequence based typing and RSCA methods. MHC can also be performed in canine and equine species
• RSCA genotyping using the ABI 3100 platform is available when Sequence Based Typing (SBT) is not appropriate

Genotyping Services

Services currently provided include:

• Microsatellite genotyping using ABI3100 platform  
• SNP genotyping using Sequenom mass spectrometry iplex technology
• RSCA genotyping using ABI3100 platform