Changes in the mechanical properties of the ageing and diseased heart severely impact ventricular compliance and hence cardiac function. Although the giant elastic protein titin is the major determinant of passive tension in cardiac myocytes, it is not known if changes in the nano-mechanical properties of this protein occur in the failing and ageing heart.
This work, carried out in collaboration with colleagues in the Institute of Cardiovascular Sciences, aims to determine if:
- Titin structure is altered in ageing and diseased heart
- Changes in titin structure directly influence titin extensibility
- Graham, H.K., Sherratt, M.J., Trafford, A.W. 2009. The Mechanical Properties of Titin Molecules Isolated from the Failing Heart. J. Gen. Physiol. 134,2A-3A (2009)
Graham, H.K., Sherratt, M.J., Watson, R.E.B. Probing the mechanical properties of cardiac titin by molecular combing and atomic force microscopy. Proc Life Sciences PC328 (2007)