Metabolomics Laboratory: Instruments

Metabolic profiling

LECO GC-TOF-MS system (Agilent 7890 coupled with a LECO Pegasus HT TOF mass spectrometer)
 

ThermoFisher UHPLC-MS (ThermoFisher Accela UHPLC coupled to ThermoFisher Orbitrap Velos)

Targeted analysis

ThermoFisher UHPLC-Triple Quadrupole-MS system (ThermoFisher Accela UHPLC coupled to ThermoFisher TSQ Vantage)

Data processing software

  • ChromaTof software for GC-MS raw data processing and data visualisation (LECO)
  • Xcalibur software for UHPLC-MS raw data processing and data visualisation (ThermoFisher Scientific)
  • Mass Frontier for UHPLC-MS in-silico fragmentation and mass spectral library searching (HighChem)
  • SIEVE for UHPLC-MS raw data processing (ThermoFisher Scientific)
  • XCMS for UHPLC-MS raw data processing (developed in Scripps Center for Metabolomics)
  • SIMPCA-Q for multivariate data analysis (Umetrics)

Sample preparation

  • QIAGEN TissueLyserII
  • Savant SPD131DDA vacuum concentrator
  • Eppendorf Concentrator plus vacuum concentrators
  • Beckman Coulter Optima L-100K ultra centrifuge and Avanti J-E centrifuge